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Assisted hatching through micromanipulation has been shown to increase the
implantation rate of replaced embryos. Certain patients may significantly
benefit from assisted hatching based on age and infertility type. Even
if sperm egg interaction and embryonic cleavage proceed normally, attachment
of the pre-embryo to the uterus must still occur.
A layer covering the ooctye called the zona pellucida performs many crucial
functions in the scheme of fertilization and development. However, in
order for the embryo to implant into the uterine wall, is must hatch
out from the zona pellucida with the help of substances produced
by the embryo.
A number of findings suggest that human IVF pre-embryos sometimes lack
the ability to thin and hatch through their zona pellucida. The
assisted hatching procedure utilized in the ART laboratory is
called zona drilling. During this process, the pre-embryo is
held by a micropipette while an acidic solution (Acid Tyrode's
Buffer) is administered to the surface of the zona pellucida
with a microneedle. The process is microscopically observed
and carefully controlled. After hatching is achieved, the
pre-embryo is returned to fresh culture media in preparation
for pre-embryo transfer.
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